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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in several tumors, which leads to chromosome instability, and thus could lead to tumorigenesis. This protein was located to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial position in endorsing mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however available.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide ordinarily consists of the epitope identified by the antibody.
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This guide outlines further more attainable optimization of mobile and immunoassay parameters to make certain the best possible results are...
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This manual outlines more achievable optimization of cellular and immunoassay parameters to make certain the absolute best benefits are...
Antibodies certain to the blocking peptide no longer bind for the epitope within the goal protein. By evaluating the staining with the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Details Cyclin E belongs for the extremely conserved cyclin family members, whose associates are characterised by a spectacular periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E forms a posh with and features being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S period boundary and it is degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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